Molecular characterization of pestiviruses in fetal bovine sera originating from Argentina: evidence of circulation of HoBi-like viruses
Serological evidence suggests that HoBi-like viruses, an emerging species within the Pestivirus genus of the Flaviviridae family, are in circulation in Argentina. While HoBi-like viruses were first isolated from Brazilian fetal bovine serum (FBS), no survey of Argentine FBS has been conducted. Therefore, 124 local samples of non-irradiated FBS originating from Argentina were surveyed for the presence of pestiviruses using RT-PCR. Amplicons from pestivirus positive samples were genotyped. Four samples were positive for HoBi virus-specific RT-PCR, while the BVDV-positive samples (n = 45) were classified as BVDV-1b (82.2%), BVDV-1a (13.3%), and BVDV-2 (4.5%). Virus isolation and serological profile assessment were performed for the four HoBi-positive FBS lots. These results confirm the circulation of HoBi-like virus in some regions of the Argentinean territory, highlighting the need to review the diagnostic techniques currently used in the clinical cases suspected of BVDV and in contamination control protocols for adventitious agents in cells and biotechnological products.
| Main Authors: | , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | eng |
| Published: |
Frontiers Media
2019-10
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| Subjects: | Pestivirus, Técnicas de Diagnosis, Encuestas, Argentina, Biología Molecular, Pestivirus de la Diarrea Bovina, Diagnostic Techniques, Surveys, Molecular Biology, Bovine Diarrhoea Pestivirus, Molecular Characterization, Caracterización Molecular, |
| Online Access: | http://hdl.handle.net/20.500.12123/6691 https://www.frontiersin.org/articles/10.3389/fvets.2019.00359/full https://doi.org/10.3389/fvets.2019.00359 |
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