Detection of bovine herpesvirus 2 and bovine herpesvirus 4 DNA in trigeminal ganglia of naturally infected cattle by polymerase chain reaction

Establishment of latent infection within specific tissues in the host is a common biological feature of the herpesviruses. In the case of bovine herpesvirus 2 (BoHV-2), latency is established in neuronal tissues, while bovine herpesvirus 4 (BoHV-4) and ovine herpesvirus 2 (OvHV-2) latent virus targets on cells of the monocytic lineage. This study was conducted in quest of BoHV-2, BoHV-4 and OvHV-2 DNA in two hundred trigeminal ganglia (TG) specimens, derived from one hundred clinically healthy cattle, majority of them naturally infected with bovine herpesvirus 1 (BoHV-1) and bovine herpesvirus 5 (BoHV-5). Total DNA extracted from ganglia was analyzed by polymerase chain reaction (PCR) designed to amplify part of the genes coding for BoHV-2, and BoHV-4 glycoprotein B and, for OvHV-2, the gene coding for phosphoribosylformylglycinamidine synthase-like protein. BoHV-2 DNA was detected in TG samples of two (2%) and BoHV-4 DNA in nine (9%) of the animals, whereas OvHV-2 DNA could not be detected in any of the TG DNA. The two animals in which BoHV-2 DNA was identified were also co-infected with BoHV-1 and BoHV-5. Within the nine animals in which BoHV-4 DNA was detected, six were also co-infected with BoHV-1 and BoHV-5. This report provides for the first time evidence that viral DNA from BoHV-2 and BoHV-4 can be occasionally detected in TG of naturally infected cattle. Likewise, in this report we provided for the first time evidence that the co-infection of cattle with three distinct bovine herpesviruses might be a naturally occurring phenomenon.

Bibliographic Details

Main Authors:	Campos, Fabrício Souza, Franco, Ana Cláudia, Oliveira, Martha Trindade, Firpo, R.M., Strelczuk, G., Fontoura, F.E., Kulma, Marcos Iuri Roos, Maidana, Silvina Soledad, Romera, Sonia Alejandra, Spilki, Fernando, Silva, Alessandra D. Avila, Hübner, Sílvia De Oliveira, Roehe, Paulo Michel
Format:	info:ar-repo/semantics/artículo biblioteca
Language:	eng
Published:	Elsevier 2014-06-25
Subjects:	Enfermedades de los Animales, Herpes Virus Bovino, Ganado Bovino, Diagnóstico, Infecciones Latentes, ADN, Animal Diseases, Bovine Herpesvirus, Cattle, Diagnosis, Latent Infections, DNA,
Online Access:	https://www.sciencedirect.com/science/article/pii/S037811351400162X http://hdl.handle.net/20.500.12123/4345 https://doi.org/10.1016/j.vetmic.2014.03.012
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