Rapid and cost-effective process based on insect larvae for scale-up production of SARS-COV-2 spike protein for serological COVID-19 testing
Serology testing for COVID-19 is important in evaluating active immune response against SARS-CoV-2, studying the antibody kinetics, and monitoring reinfections with genetic variants and new virus strains, in particular, the duration of antibodies in virus-exposed individuals and vaccine-mediated immunity. In this study, recombinant S protein of SARS-CoV-2 was expressed in Rachiplusia nu, an important agronomic plague. One gram of insect larvae produces an amount of S protein sufficient for 150 determinations in the ELISA method herein developed. We established a rapid production process for SARS-CoV-2 S protein that showed immunoreactivity for anti-SARS-CoV-2 antibodies and was used as a single antigen for developing the ELISA method with high sensitivity (96.2%) and specificity (98.8%). Our findings provide an efficient and cost-effective platform for large-scale S protein production, and the scale-up is linear, thus avoiding the use of complex equipment like bioreactors.
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | info:ar-repo/semantics/artículo biblioteca |
| Language: | eng |
| Published: |
Wiley
2021-10
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| Subjects: | COVID-19, Coronavirus del Síndrome Respiratorio Agudo Grave 2, Ensayo, Larvas de Insectos, Proteínas, Severe Acute Respiratory Syndrome Coronavirus 2, Testing, Insect Larvae, Proteins, SARS-CoV-2, |
| Online Access: | http://hdl.handle.net/20.500.12123/10759 https://onlinelibrary.wiley.com/doi/full/10.1002/bit.27889 https://doi.org/10.1002/bit.27889 |
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