Growth and morphogenesis of shoot initials of Douglas fir, Pseudotsuga menziesii (Mirb.) Franco, in vitro
An optimalized method of micropropagation of Douglas fir is described. Seasonal changes were found in optima for nitrate and sucrose in the medium and in the optimum for the light intensity during the culture of shoot initials. Differences in morphogenesis were obtained from shoot initials that had been isolated from buds in 10 topophysical positions on 2-year-old trees. These differences between the shoots were influenced by the medium, the light intensity, and by forcing or topping the mother trees or treating them with growth regulators. Shoots from each of the topophysical positions had a characteristic photosynthesis. A first attempt is made to compare in vivo flushing with the morphogenesis of shoot initials in vitro.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Doctoral thesis biblioteca |
| Language: | English |
| Published: |
Evers
|
| Subjects: | asexual reproduction, buds, environmental factors, forestry, forestry practices, growth, in vitro culture, plant morphology, pseudotsuga menziesii, shoots, silviculture, stems, trees, vegetative propagation, bomen, bosbouw, bosbouwkundige handelingen, groei, houtteelt, in vitro kweek, knoppen, milieufactoren, ongeslachtelijke voortplanting, plantenmorfologie, scheuten, stengels, vegetatieve vermeerdering, |
| Online Access: | https://research.wur.nl/en/publications/growth-and-morphogenesis-of-shoot-initials-of-douglas-fir-pseudot-4 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|