Amplicon sequencing of selected H. schachtii loci following lipofection of CRISPR reagents

Amplicon sequencing of selected H. schachtii loci following lipofection of CRISPR reagents

Second stage juvenile Heterodera schachtii were soaked in liposomes containing CRISPR Cas9 components targeting eleven genes, with the goal of introducing edits by non-homologous end joining or homology directed repair.

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Bibliographic Details
Main Authors: Kranse, Olaf, Beasley, Helen, Adams, Sally, Pires-Da Silva, Andre, Bell, Christopher, Lilley, Catherine J., Urwin, Peter E., Bird, David, Miska, Eric, Smant, Geert, Gheysen, Godelieve, Jones, John, Viney, Mark, Abad, Pierre, Maier, Thomas R., Baum, Thomas J., Siddique, Shahid, Williamson, Valerie, Akay, Alper, Eves-Van Den Akker, Sebastian
Format: Dataset biblioteca
Published: University of Cambridge
Subjects:Amplicon sequencing, CRISPR Cas9, Heterodera schachtii, Lipofection, Liposome,
Online Access:https://research.wur.nl/en/datasets/amplicon-sequencing-of-selected-h-schachtii-loci-following-lipofe
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https://research.wur.nl/en/datasets/amplicon-sequencing-of-selected-h-schachtii-loci-following-lipofe

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