Successional dynamics in the gut microbiome determine the success of Clostridium difficile infection in adult pig models.
Clostridium difficile infections (CDI) are a major cause of antibiotic-associated diarrhea. It is</p><p>hypothesized that CDI develops due to the antibiotic-induced disruption of the intestinal</p><p>microbial community structure, which allows C. difficile to flourish. Here, we pre-treated</p><p>weaned pigs with the antibiotics Clindamycin or Ciprofloxacin for one day, and subsequently</p><p>inoculated them with a human and pig enteropathogenic C. difficile strain 078 spores. Body</p><p>temperature, clinical signs of disease, and the fecal microbiome were monitored daily for 15</p><p>days. Clindaycin had a stronger effect on the pigs than Ciprofloxacin, resulting in drastic shifts in</p><p>the fecal microbiome, decreases in microbial diversity and significant increases in body</p><p>temperature, even in the absence of C. difficile. Fecal shedding of C. difficile was detectable for 3</p><p>and 9 days in Ciprofloxacin and Clindamycin treated pigs inoculated with C. difficile,</p><p>respectively, and in both cases decreased cell proliferation rates were detected in colon tissue.</p><p>The timing of C. difficile shedding coincided with the decrease in a large cluster of Firmicutes</p><p>following Clindamycin treatment, a pattern which was also independent of C. difficile</p><p>inoculation. The observed community dynamics suggest that competitive interactions following</p><p>antibiotic treatment facilitate C. difficile establishment.
| Main Authors: | , , , , |
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| Format: | Dataset biblioteca |
| Published: |
Wageningen University & Research
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| Subjects: | Multispecies, pig gut metagenome, weaned pig, |
| Online Access: | https://research.wur.nl/en/datasets/successional-dynamics-in-the-gut-microbiome-determine-the-success |
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