Haplotype-resolved assembly of a tetraploid potato genome using long reads and low-depth offspring data

Potato is one of the world’s major staple crops, and like many important crop plants, it has a polyploid genome. Polyploid haplotype assembly poses a major computational challenge. We introduce a novel strategy for the assembly of polyploid genomes and present an assembly of the autotetraploid potato cultivar Altus. Our method uses low-depth sequencing data from an offspring population to achieve chromosomal clustering and haplotype phasing on the assembly graph. Our approach generates high-quality assemblies of individual chromosomes with haplotype-specific sequence resolution of whole chromosome arms and can be applied in common breeding scenarios where collections of offspring are available.

Bibliographic Details

Main Authors:	Serra Mari, Rebecca, Schrinner, Sven, Finkers, Richard, Ziegler, Freya Maria Rosemarie, Arens, Paul, Schmidt, Maximilian H.W., Usadel, Björn, Klau, Gunnar W., Marschall, Tobias
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	Life Science,
Online Access:	https://research.wur.nl/en/publications/haplotype-resolved-assembly-of-a-tetraploid-potato-genome-using-l
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