Replacing the Calvin cycle with the reductive glycine pathway in Cupriavidus necator

These data belong to a metabolic engineering project that introduces the reductive glycine pathway for formate assimilation in Cupriavidus necator. As part of this project we performed short-term evolution of the bacterium Cupriavidus necator H16 to grow on glycine as sole carbon and energy source. Some mutations in a putiative glycine transporting systems facilitated growth, and we performed transcriptomics on the evolved strain growing on glycine. Analysis of these transcriptomic data lead us to the discovery of a glycine oxidase (DadA6), which we experimentally demonstrated to play a key role in the glycine assimilation pathay in C. necator. Overall design: Transcriptomes are performed for the evolved strain growing on glyine (in triplicate).

Bibliographic Details

Main Authors:	Claassens, Nico J., Bordanaba-Florit, Guillermo, Cotton, Charles A.R., De Maria, Alberto, Finger-Bou, Max, Friedeheim, Lukas, Giner-Laguarda, Natalia, Munar-Palmer, Martí, Newell, William, Scarinci, Giovanni, Verbunt, Jari, de Vries, Stijn T., Yilmaz, Suzan, Bar-Even, Arren
Format:	Dataset biblioteca
Published:	Wageningen University & Research
Subjects:	Cupriavidus necator,
Online Access:	https://research.wur.nl/en/datasets/replacing-the-calvin-cycle-with-the-reductive-glycine-pathway-in-
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