X-ray Computed Tomography (CT) Scanning Is a Non-Destructive and Modern Technique to Identify and Assess the Characteristics of Armillaria solidipes Pathogen Infections in Poplar Roots
(1) Objective: The opacity of soils complicates studies of root infection. An example of this is the infection of Armillaria solidipes on poplar (Populus davidiana × Populus alba var. pyramidalis Louche) roots systems, which risks damaging trees. (2) Methods: Only one of the four tested substrates for tree species was shown to be suitable to perform X-ray computed tomography (CT). Three-dimensional (3D) imaging was used to reconstruct the root system of poplar seedlings and the changes caused by the infection. (3) Results: We developed a protocol to efficiently grow poplar on a synthetic matrix, vermiculite, that allows for monitoring the root system by X-ray CT. Poplar 3D reconstruction of the root system was automated using the software Win-RHIZO, and various infection parameters were identified. (4) Conclusions: Our procedure allows for monitoring the infection of root systems and provides new opportunities to characterize the complex Armillaria solidipes poplar interaction using X-ray CT.
| Main Authors: | , , , , , |
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | Life Science, |
| Online Access: | https://research.wur.nl/en/publications/x-ray-computed-tomography-ct-scanning-is-a-non-destructive-and-mo |
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