Extending the diversity of Myceliophthora thermophila LPMOs : Two different xyloglucan cleavage profiles
Lytic polysaccharide monooxygenases (LPMOs) play a key role in enzymatic conversion of plant cell wall polysaccharides. Continuous discovery and functional characterization of LPMOs highly contribute to the tailor-made design and improvement of hydrolytic-activity based enzyme cocktails. In this context, a new MtLPMO9F was characterized for its substrate (xyloglucan) specificity, and MtLPMO9H was further delineated. Aided by sodium borodeuteride reduction and hydrophilic interaction chromatography coupled to mass spectrometric analysis, we found that both MtLPMOs released predominately C4-oxidized, and C4/C6-double oxidized xylogluco-oligosaccharides. Further characterization showed that MtLPMO9F, having a short active site segment 1 and a long active site segment 2 (−Seg1+Seg2), followed a “substitution-intolerant” xyloglucan cleavage profile, while for MtLPMO9H (+Seg1−Seg2) a “substitution-tolerant” profile was found. The here characterized xyloglucan specificity and substitution (in)tolerance of MtLPMO9F and MtLPMO9H were as predicted according to our previously published phylogenetic grouping of AA9 LPMOs based on structural active site segment configurations.
| Main Authors: | , , , |
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | Active site segment, LPMOs, Lignocellulose, Mass spectrometric fragmentation, Oxidative cleavage, Reduction, Xyloglucan, |
| Online Access: | https://research.wur.nl/en/publications/extending-the-diversity-of-myceliophthora-thermophila-lpmos-two-d |
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