Actinidin-induced hydrolysis of milk proteins : Effect on antigenicity
Actinidin was used to hydrolyse proteins in whey protein isolate (WPI) and milk protein concentrate (MPC) to reduce immunoreactivity of β-lactoglobulin (β-LG) and αs1-casein (αs1-CN). Samples were incubated at an enzyme to substrate ratio of 1:100 (5.21 units of actinidin activity g−1 of protein) at 10 or 60 °C for up to 31 or 5 h, respectively. Protein hydrolysis was determined by the degree of hydrolysis and SDS-PAGE. Antigenicity of hydrolysates was determined by β-LG and αs1-CN antibody-binding capacity using enzyme-linked immunosorbent assay (ELISA) quantification kits. ELISA showed significant reduction of antigenicity of β-LG and αs1-CN with higher degree of hydrolysis (DH) by actinidin. At 60 °C, hydrolysis for 5 h resulted in antigenicity reduction of ∼43% for β-LG and ∼48% for αs1-CN in MPC and ∼54% for β-LG in WPI. Hydrolysis at 10 °C for 31 h also resulted in decrease in antigenicity in MPC for β-LG and αs1-CN by ∼39 and 42% respectively, but only 14% for β-LG in WPI. Treatment with actinidin can reduce the antigenicity by modification of protein conformation and cleavage and masking of epitopes of β-LG and αs1-CN.
| Main Authors: | , , |
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | Actinidin, Antigenicity, Proteolysis, αs-casein, β-lactoglobulin, |
| Online Access: | https://research.wur.nl/en/publications/actinidin-induced-hydrolysis-of-milk-proteins-effect-on-antigenic |
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| Summary: | Actinidin was used to hydrolyse proteins in whey protein isolate (WPI) and milk protein concentrate (MPC) to reduce immunoreactivity of β-lactoglobulin (β-LG) and αs1-casein (αs1-CN). Samples were incubated at an enzyme to substrate ratio of 1:100 (5.21 units of actinidin activity g−1 of protein) at 10 or 60 °C for up to 31 or 5 h, respectively. Protein hydrolysis was determined by the degree of hydrolysis and SDS-PAGE. Antigenicity of hydrolysates was determined by β-LG and αs1-CN antibody-binding capacity using enzyme-linked immunosorbent assay (ELISA) quantification kits. ELISA showed significant reduction of antigenicity of β-LG and αs1-CN with higher degree of hydrolysis (DH) by actinidin. At 60 °C, hydrolysis for 5 h resulted in antigenicity reduction of ∼43% for β-LG and ∼48% for αs1-CN in MPC and ∼54% for β-LG in WPI. Hydrolysis at 10 °C for 31 h also resulted in decrease in antigenicity in MPC for β-LG and αs1-CN by ∼39 and 42% respectively, but only 14% for β-LG in WPI. Treatment with actinidin can reduce the antigenicity by modification of protein conformation and cleavage and masking of epitopes of β-LG and αs1-CN. |
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