First structural insights into CRISPR-Cas-guided DNA transposition
The transposition mechanism of the Vibrio cholerae Tn6677 transposon (VcTn6677) is based on the unique synergy between a classical transposition machinery (TnsABC-TniQ complex) and a nuclease-deficient type I-F CRISPR-associated complex for antiviral defense (VcCascade). Four independent studies, three of which appeared in Cell Research, recently reported structures of the VcCascade, VcCascade-TniQ, and/or VcCascade-TniQ-dsDNA complexes as well as a TniQ dimer complex, providing the first insights into the assembly and DNA targeting mechanism of a CRISPR-associated transposase complex.
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| Main Authors: | , |
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | Life Science, |
| Online Access: | https://research.wur.nl/en/publications/first-structural-insights-into-crispr-cas-guided-dna-transpositio |
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