Enhanced biosynthesis of the natural antimicrobial glyceollins in soybean seedlings by priming and elicitation

Glyceollins are a class of antimicrobial prenylated pterocarpans produced in soybean seedlings upon fungus elicitation. Priming with reactive oxygen species (ROS) prior to elicitation with Rhizopus oligosporus/oryzae (R) was investigated for its potential to enhance glyceollin production. ROS-priming prior to R-elicitation (ROS + R) increased glyceollin production (8.6 ± 0.9 µmol/g dry weight (DW)) more than 4-fold compared to elicitation without priming (1.9 ± 0.4 µmol/g DW). Furthermore, ROS-priming was superior to two physical primers which were used as benchmark primers, namely slicing (5.0 ± 0.6 µmol glyceollins/g DW) and sonication (4.8 ± 1.0 µmol glyceollins/g DW). Subsequently, the robustness of ROS + R was assessed by applying it to another soybean cultivar, where it also resulted in a significantly higher glyceollin content than R-elicitation without priming. ROS-priming prior to elicitation provides opportunities for improving the yield in large-scale production of natural antimicrobials due to the ease of application and the robustness of the effect across cultivars.

Bibliographic Details

Main Authors:	Kalli, Sylvia, Araya-Cloutier, Carla, Lin, Yiran, de Bruijn, Wouter J.C., Chapman, John, Vincken, Jean Paul
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	Elicitation, Fungus, Glyceollins, Priming, Reactive oxygen species, Soybeans,
Online Access:	https://research.wur.nl/en/publications/enhanced-biosynthesis-of-the-natural-antimicrobial-glyceollins-in
Tags:	Add Tag No Tags, Be the first to tag this record!