Direct target gene analyses for TCP20 using a glucocorticoid inducible TCP20 (TCP20-GR)

TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PCF1) transcription factors control developmental processes in plants. We identified direct target genes of the Arabidopsis class I TCP20 protein in leaf development based on a glucocorticoid receptor induction assay and genome-wide expression studies. For this, we tagged TCP20 with a glucucorticoid receptor (GR) domain and transformed the resulting TCP20-GR construct into tcp20 knockout plants. Induction of these and wild type controls was done with Dexamethasone and Cycloheximide. Plants were harvested 8 h after induction, uninduced plants were taken as control.

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Bibliographic Details
Main Authors: Danisman, S.D., van der Wal, F., de Folter, S., Naouar, N., Muino Acuna, J.M., Angenent, G.C., Immink, G.H.
Format: Dataset biblioteca
Published: Plant Research International
Subjects:Arabidopsis thaliana,
Online Access:https://research.wur.nl/en/datasets/direct-target-gene-analyses-for-tcp20-using-a-glucocorticoid-indu
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