Dilute H2SO4-catalyzed hydrothermal pretreatment to enhance enzymatic digestibility of Jatropha curcas fruit hull for ethanol fermentation
Dilute sulfuric acid pretreatment of the Jatropha curcas fruit hull at high temperatures (140°C to 180°C) performed in a 110-mL stainless steel reactor was investigated to enhance the enzymatic digestibility of its lignocellulosic components. Carbohydrates accounted for 43% of the dry matter of the J. curcas fruit hull biomass. The goal of the study was to optimize the pretreatment conditions (acid concentration, time, and temperature) in order to obtain the highest sugar yield after subsequent enzymatic hydrolysis. A Box-Behnken design was applied to the experimental setup in order to reduce the number of experiments. The optimal pretreatment conditions are 30-min incubations at a temperature of 178°C with a sulfuric acid concentration of 0.9% (w/v). Using these pretreatment conditions for a fruit solid loading of 9.52% followed by a 24-h enzymatic hydrolysis resulted in a liberation of 100% of all pentoses present (71% yield and 29% degradation to furfural) and 83% of the hexoses (78% yield and 5% degradation to 5-hydroxymethylfurfural). The simultaneous saccharification and fermentation experiment showed that acid-pretreated fruit hull can be used as a substrate for Saccharomyces cerevisiae to produce ethanol.
| Main Authors: | , , , |
|---|---|
| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | Box-Behnken, Cellulose, Ethanol, Fruit hull, Hemicellulose, Hexose, Jatropha curcas, Pentose, |
| Online Access: | https://research.wur.nl/en/publications/dilute-h2so4-catalyzed-hydrothermal-pretreatment-to-enhance-enzym |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|