Analysis of omega-3 fatty acid derived N-acylethanolamines in biological matrices
The adequate quantification of endocannabinoids can be complex due to their low endogenous levels and structural diversity. Therefore, advanced analytical approaches, such as LC-MS, are used to measure endocannabinoids in plasma, tissues, and other matrices. Recent work has shown that endocannabinoids that are synthesized from n-3 fatty acids, such as docosahexaenoylethanolamide (DHEA) and eicosapentaenoylethanolamide (EPEA), have anti-inflammatory and anti-tumorigenic properties and stimulate synapse formation in neurites. Here, an LC-MS based method for the quantification of n-3 endocannabinoids DHEA and EPEA which is also suited to measure a wider spectrum of endocannabinoids is described. The chapter contains a step-by-step protocol for the analysis of n-3 endocannabinoids in plasma, including sample collection and solid phase extraction, LC-MS analysis, and data processing. Modifications to the protocol that allow quantifying n-3 endocannabinoids in tissues and cell culture media will also be discussed. Finally, conditions that alter endocannabinoid concentrations are briefly discussed.
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| Format: | Part of book or chapter of book biblioteca |
| Language: | English |
| Published: |
Humana Press
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| Subjects: | Docosahexaenoylethanolamide, Eicosapentaenoylethanolamide, Endocannabinoids, LC-MS, N-3 fatty acids, Solid phase extraction, |
| Online Access: | https://research.wur.nl/en/publications/analysis-of-omega-3-fatty-acid-derived-n-acylethanolamines-in-bio |
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