Transient-state kinetic analysis of complex formation between photoprotein clytin and GFP from jellyfish Clytia gregaria
Luminous organisms use different protein-mediated strategies to modulate light emission color. Here, we report the transient-state kinetic studies of the interaction between photoprotein clytin from Clytia gregaria and its antenna protein, cgreGFP. We propose that cgreGFP forms a transient complex with Ca2+-bound clytin before the excited singlet state of the coelenteramide product is formed. From the spectral distribution and donor-acceptor separation distance, we infer that clytin reaction intermediates may interact only with the middle side part of cgreGFP.
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | FRET, aequorin, bioluminescence, coelenterazine, obelin, protein-protein interaction, |
| Online Access: | https://research.wur.nl/en/publications/transient-state-kinetic-analysis-of-complex-formation-between-pho |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|