Knockout of the Bcmo1 gene results in an inflammatory response in female lung, which is suppressed by dietary beta-carotene

Beta-carotene 15,15'-monooxygenase 1 knockout (Bcmo1 (-/-)) mice accumulate beta-carotene (BC) similarly to humans, whereas wild-type (Bcmo1 (+/+)) mice efficiently cleave BC. Bcmo1 (-/-) mice are therefore suitable to investigate BC-induced alterations in gene expression in lung, assessed by microarray analysis. Bcmo1 (-/-) mice receiving control diet had increased expression of inflammatory genes as compared to BC-supplemented Bcmo1 (-/-) mice and Bcmo1 (+/+) mice that received either control or BC-supplemented diets. Differential gene expression in Bcmo1 (-/-) mice was confirmed by real-time quantitative PCR. Histochemical analysis indeed showed an increase in inflammatory cells in lungs of control Bcmo1 (-/-) mice. Supported by metabolite and gene-expression data, we hypothesize that the increased inflammatory response is due to an altered BC metabolism, resulting in an increased vitamin A requirement in Bcmo1 (-/-) mice. This suggests that effects of BC may depend on inter-individual variations in BC-metabolizing enzymes, such as the frequently occurring human polymorphisms in BCMO1.

Bibliographic Details

Main Authors:	Helden, Y.G.J., Heil, S.G., van Schooten, F.J., Kramer, E., Hessel, S., Amengual, J., Ribot, J., Teerds, K.J., Wyss, A., Lietz, G., Bonet, M.L., von Lintig, J., Godschalk, R.W.L., Keijer, J.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	acid, alcohol-dehydrogenase, cancer, cardiovascular-disease, cells, double-tracer, mice, nonsteroidal antiinflammatory drugs, retinol efficacy trial, vitamin-a-deficiency,
Online Access:	https://research.wur.nl/en/publications/knockout-of-the-bcmo1-gene-results-in-an-inflammatory-response-in
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