Glycoalkaloids selectively permeabilize cholesterol containing biomembranes.

The effects of the glycoalkaloids α-solanine, α-chaconine and α-tomatine on different cell types were studied in order to investigate the membrane action of these compounds. Hemolysis of erythrocytes was compared to 6-carboxyfluorescein leakage from both ghosts and erythrocyte lipid vesicles, whereas leakage of enzymes from mitochondria and the apical and baso-lateral side of Caco-2 cells was determined. Furthermore, the effects of glycoalkaloids on the gap-junctional communication between Caco-2 cells was studied. From these experiments, it was found that glycoalkaloids specifically induced membrane disruptive effects of cholesterol containing membranes as was previously reported in model membrane studies. In addition, α-chaconine was found to selectively decrease gap-junctional intercellular communication. Furthermore, the glycoalkaloids were more potent in permeabilizing the outer membrane of mitochondria compared to digitonin at the low concentrations used.

Bibliographic Details

Main Authors:	Keukens, E.A.J., de Vrije, T., Jansen, L.A.M., de Boer, H., Janssen, M., de Kroon, A.I.P.M., Jongen, W.M.F., de Kruijff, B.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	Caco-2, Cholesterol, Erythrocyte, Glycoalkaloid, Membrane disruption, Mitochondrion, Permeabilization,
Online Access:	https://research.wur.nl/en/publications/glycoalkaloids-selectively-permeabilize-cholesterol-containing-bi
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