Isolation of recombinant antibodies (scFvs) to grapevine virus B
A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.
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| Main Authors: | , , , , , |
|---|---|
| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | diagnosis, elisa, fusion proteins, phage display libraries, reagents, |
| Online Access: | https://research.wur.nl/en/publications/isolation-of-recombinant-antibodies-scfvs-to-grapevine-virus-b |
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