Isolation of recombinant antibodies (scFvs) to grapevine virus B

A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.

Bibliographic Details

Main Authors:	Saldarelli, P., Keller, H.J.H.G., Dell'Orco, M., Schots, A., Elicio, V., Minafra, A.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	diagnosis, elisa, fusion proteins, phage display libraries, reagents,
Online Access:	https://research.wur.nl/en/publications/isolation-of-recombinant-antibodies-scfvs-to-grapevine-virus-b
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