Isolation of recombinant antibodies (scFvs) to grapevine virus B
A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.
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| Format: | Article/Letter to editor biblioteca |
| Language: | English |
| Subjects: | diagnosis, elisa, fusion proteins, phage display libraries, reagents, |
| Online Access: | https://research.wur.nl/en/publications/isolation-of-recombinant-antibodies-scfvs-to-grapevine-virus-b |
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dig-wur-nl-wurpubs-3371142025-01-23 Saldarelli, P. Keller, H.J.H.G. Dell'Orco, M. Schots, A. Elicio, V. Minafra, A. Article/Letter to editor Journal of Virological Methods 124 (2005) 1-2 ISSN: 0166-0934 Isolation of recombinant antibodies (scFvs) to grapevine virus B 2005 A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis. en application/pdf https://research.wur.nl/en/publications/isolation-of-recombinant-antibodies-scfvs-to-grapevine-virus-b 10.1016/j.jviromet.2004.11.021 https://edepot.wur.nl/44089 diagnosis elisa fusion proteins phage display libraries reagents Wageningen University & Research |
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diagnosis elisa fusion proteins phage display libraries reagents diagnosis elisa fusion proteins phage display libraries reagents Saldarelli, P. Keller, H.J.H.G. Dell'Orco, M. Schots, A. Elicio, V. Minafra, A. Isolation of recombinant antibodies (scFvs) to grapevine virus B |
| description |
A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis. |
| format |
Article/Letter to editor |
| topic_facet |
diagnosis elisa fusion proteins phage display libraries reagents |
| author |
Saldarelli, P. Keller, H.J.H.G. Dell'Orco, M. Schots, A. Elicio, V. Minafra, A. |
| author_facet |
Saldarelli, P. Keller, H.J.H.G. Dell'Orco, M. Schots, A. Elicio, V. Minafra, A. |
| author_sort |
Saldarelli, P. |
| title |
Isolation of recombinant antibodies (scFvs) to grapevine virus B |
| title_short |
Isolation of recombinant antibodies (scFvs) to grapevine virus B |
| title_full |
Isolation of recombinant antibodies (scFvs) to grapevine virus B |
| title_fullStr |
Isolation of recombinant antibodies (scFvs) to grapevine virus B |
| title_full_unstemmed |
Isolation of recombinant antibodies (scFvs) to grapevine virus B |
| title_sort |
isolation of recombinant antibodies (scfvs) to grapevine virus b |
| url |
https://research.wur.nl/en/publications/isolation-of-recombinant-antibodies-scfvs-to-grapevine-virus-b |
| work_keys_str_mv |
AT saldarellip isolationofrecombinantantibodiesscfvstograpevinevirusb AT kellerhjhg isolationofrecombinantantibodiesscfvstograpevinevirusb AT dellorcom isolationofrecombinantantibodiesscfvstograpevinevirusb AT schotsa isolationofrecombinantantibodiesscfvstograpevinevirusb AT eliciov isolationofrecombinantantibodiesscfvstograpevinevirusb AT minafraa isolationofrecombinantantibodiesscfvstograpevinevirusb |
| _version_ |
1822274390872555520 |