Validation of three rapid screening methods for detection of verotoxin-producing Escherichia coli in foods : interlaboratory study

An interlaboratory study was conducted for the validation of 3 methods for the detection of all verotoxin-producing Escherichia coli (VTEC) in foods. The methods were a multi-analyte 1-step lateral flow immunoassay (LFIA) for detection of E. coli O157 and verotoxin (VT); an enzyme-linked immunosorbent assay targeted against VT1, VT2, and VT2c (VT-ELISA); and a polymerase chain reaction (PCR) method for detection of VT genes (VT-PCR). Aliquots (25 g or 25 mL) of 4 food types (raw minced [ground] beef, unpasteurized milk, unpasteurized apple juice [cider], and salami) were individually inoculated with low numbers (

Bibliographic Details

Main Authors:	Capps, K.L., McLaughlin, E.M., Murray, A.W.A., Aldus, C.F., Wyatt, G.M., Peck, M.W., van Amerongen, A., Ariens, R.M.C., Wichers, J.H., Bayliss, C.L., Wareing, D.R.A., Bolton, F.J.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	england, o157, vtec, wales,
Online Access:	https://research.wur.nl/en/publications/validation-of-three-rapid-screening-methods-for-detection-of-vero
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