Transposon tagging : towards the isolation of the resistance R-genes in potato against Phytophthora infestans (Mont.) de Bary
The thesis describes the first step of the research leading to transposon tagging of the resistance genes against late blight of potato (Phytophthora infestans). Different diploid potato populations have been developed in which four R -genes have been localized on the RFLP map. Screening for high regeneration and transformation competence in a segregating population showed positive correlation between the R1 gene and the competence for transformation. It resulted also in the selection of a diploid potato clone suitable for transposon tagging. The transformation of this clone with Ds -carrying T- DNA and the localization of 60 different T-DNA insertions were carried out. The involvement of other genetic factors in the expression of the R -genes was detected.
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| Format: | Doctoral thesis biblioteca |
| Language: | English |
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Landbouwuniversiteit Wageningen
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| Subjects: | disease resistance, fungicides, genetic engineering, pest resistance, phytophthora, plant breeding, plant pathogenic fungi, plant protection, potatoes, recombinant dna, solanum tuberosum, aardappelen, fungiciden, genetische modificatie, gewasbescherming, plaagresistentie, plantenveredeling, plantenziekteverwekkende schimmels, ziekteresistentie, |
| Online Access: | https://research.wur.nl/en/publications/transposon-tagging-towards-the-isolation-of-the-resistance-r-gene |
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