Plant members of the alpha1->3/4-fucosyltransferase gene family encode an alpha1-> 4 fucosyltransferase potentially involved in Lewisa biosynthesis and two core alpha1->3-fucosyltransferases

Three putative alpha1-->3/4-fucosyltransferase (alpha1-->3/4-FucT) genes have been detected in the Arabidopsis thaliana genome. The products of two of these genes have been identified in vivo as core alpha1-->3-FucTs involved in N-glycosylation. An orthologue of the third gene was isolated from a Beta vulgaris cDNA library. The encoded enzyme efficiently fucosylates Galbeta1-->3GlcNAcbeta1-->3Galbeta1-->4Glc. Analysis of the product by 400 MHz (1)H-nuclear magnetic resonance spectroscopy showed that the product is alpha1-->4-fucosylated at the N-acetylglucosamine residue. In vitro, the recombinant B. vulgaris alpha1-->4-FucT acts efficiently only on neutral type 1 chain-based glycan structures. In plants the enzyme is expected to be involved in Lewis(a) formation on N-linked glycans.

Bibliographic Details

Main Authors:	Bakker, H., Schijlen, E., de Vries, T., Schiphorst, W.E.C.M., Jordi, W.J.R.M., Lommen, A., Bosch, H.J., van Die, I.
Format:	Article/Letter to editor biblioteca
Language:	English
Subjects:	Fucose, Fucosyltransferase, Glycosyltransferase, Lewisa,
Online Access:	https://research.wur.nl/en/publications/plant-members-of-the-alpha1-gt34-fucosyltransferase-gene-family-e
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