2D-NMR demonstration of lignin removal from wood and non-wood plant feedstocks by fungal versatile peroxidase
The ability of versatile peroxidase to modify/degrade lignin (in conjunction with veratryl alcohol as a mediator) in both wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) lignocellulosic feedstocks was demonstrated using the Pleurotus ostreatus enzyme heterologously expressed in Escherichia coli and "in vitro" folded. Two-dimensional nuclear magnetic resonance (NMR) of the treated materials, swollen in dimethylsulfoxide-d6, revealed a reduction in the number of lignin side-chain linkages after both treatments. However, a significant removal of lignin by the enzymatic treatment was only observed in the case of eucalypt wood, as shown by the decrease of lignin aromatic signals referred to carbohydrate anomeric signals.
Saved in:
Main Authors: | Fernández-Fueyo, Elena, Rencoret, Jorge, Berbís, Manuel Álvaro, Ruiz-Dueñas, F. J., Gutiérrez Suárez, Ana, Río Andrade, José Carlos del, Jiménez-Barbero, Jesús, Hammel, Kenneth E., Martínez, Ángel T. |
---|---|
Format: | capítulo de libro biblioteca |
Language: | English |
Published: |
CSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS)
2014-06-24
|
Subjects: | Lignin, Enzymes, Versatile peroxidase, Veratryl alcohol, 2D NMR, Eucalyptus, |
Online Access: | http://hdl.handle.net/10261/98704 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Mecanismos de biodegradación de la lignina
by: Gutiérrez Suárez, Ana, et al.
Published: (1996) -
Peroxidase evolution in white-rot fungi follows wood lignin evolution in plants
by: Ayuso-Fernández, Iván, et al.
Published: (2019-08-19) -
Delignification of eucalypt kraft pulp with manganese-substituted polyoxometalate assisted by fungal versatile peroxidase
by: Marques, Gisela, et al. -
How ligninolytic enzymes attack lignin: A rapid spectrophotometry and 2D NMR study
by: Rencoret, Jorge, et al.
Published: (2018-06-26) -
2D NMR study of lignin modification during pretreatment of Eucalyptus wood feedstock with a fungal laccase and a phenolic mediator
by: Rico Campos, Alejandro, et al.
Published: (2014-06-24)