2D-NMR demonstration of lignin removal from wood and non-wood plant feedstocks by fungal versatile peroxidase

The ability of versatile peroxidase to modify/degrade lignin (in conjunction with veratryl alcohol as a mediator) in both wood (Eucalyptus globulus) and non-wood (Pennisetum purpureum) lignocellulosic feedstocks was demonstrated using the Pleurotus ostreatus enzyme heterologously expressed in Escherichia coli and "in vitro" folded. Two-dimensional nuclear magnetic resonance (NMR) of the treated materials, swollen in dimethylsulfoxide-d6, revealed a reduction in the number of lignin side-chain linkages after both treatments. However, a significant removal of lignin by the enzymatic treatment was only observed in the case of eucalypt wood, as shown by the decrease of lignin aromatic signals referred to carbohydrate anomeric signals.

Bibliographic Details

Main Authors:	Fernández-Fueyo, Elena, Rencoret, Jorge, Berbís, Manuel Álvaro, Ruiz-Dueñas, F. J., Gutiérrez Suárez, Ana, Río Andrade, José Carlos del, Jiménez-Barbero, Jesús, Hammel, Kenneth E., Martínez, Ángel T.
Format:	capítulo de libro biblioteca
Language:	English
Published:	CSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS) 2014-06-24
Subjects:	Lignin, Enzymes, Versatile peroxidase, Veratryl alcohol, 2D NMR, Eucalyptus,
Online Access:	http://hdl.handle.net/10261/98704
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