The study of stable electrophoretic fractions of humic acids by pyrolytic gas chromatography-mass spectrometry
Polyacrylamide gel electrophoresis in combination with size exclusion chromatography was used for separating stable electrophoretic fractions of humic acids (HAs) from soils of different genesis (soddy-podzolic soil, chernozem, and krasnozem). The pyrolytic gas chromatography-mass spectrometry based on thermal hydrolysis with simultaneous methylation revealed the nonuniform distribution of saturated fatty acid homologues of plant origin among the electrophoretic fractions of HAs, which indicated the different biogenic origins of the fractions. The obtained results present direct experimental proof of the general principle of molecular structure of soil HAs regardless of their genesis.
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| Main Authors: | , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
MAIK Nauka/Interperiodica Publishing
2005
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| Online Access: | http://hdl.handle.net/10261/58830 |
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