Optical thermal cycler for use as a fluorimetric plate reader to estimate DNA concentrations

Recent developments in PCR techniques provide the opportunity to quantify low copy numbers of specific nucleic acid sequences by quantitative, real-time PCR (1). The use of quantitative, real-time PCR is spreading due to its accuracy and relative simplicity. As a consequence, the number of laboratories equipped with an optical thermal cycler is increasing.

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Bibliographic Details
Main Authors: González Grau, Juan Miguel, Sáiz-Jiménez, Cesáreo
Other Authors: Ministerio de Ciencia y Tecnología (España)
Format: artículo biblioteca
Language:English
Published: Eaton Publications 2003
Online Access:http://hdl.handle.net/10261/58622
http://dx.doi.org/10.13039/501100006280
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