A mutant O-GlcNAcase as a probe to reveal global dynamics of protein O-GlcNAcylation during Drosophila embryonic development

O-GlcNAcylation is a reversible type of serine/threonine glycosylation on nucleocytoplasmic proteins in metazoa. Various genetic approaches in several animal models have revealed that O-GlcNAcylation is essential for embryogenesis. However, the dynamic changes in global O-GlcNAcylation and the underlying mechanistic biology linking them to embryonic development is not understood. One of the limiting factors towards characterizing changes in O-GlcNAcylation has been the limited specificity of currently available tools to detect this modification. In the present study, harnessing the unusual properties of an O-GlcNAcase (OGA) mutant that binds O-GlcNAc (O-N-acetylglucosamine) sites with nanomolar affinity, we uncover changes in protein O-GlcNAcylation as a function of Drosophila development.

Bibliographic Details

Main Authors:	Mariappa, Daniel, Selvan, Nithya, Borodkin, Vladimir S., Jana Alonso Lorenzo, Ferenbach, Andrew T., Shepherd, Claire, Navratilova, Iva Hopkins, van Aalten, Daan M.F.
Other Authors:	Wellcome Trust Senior Research Fellowship
Format:	artículo biblioteca
Language:	English
Published:	Portland Press 2015-08-20
Subjects:	O-GlcNAcase (OGA), Fluorescence polarization, Drosophila, embryonic development, O-GlcNAc probe,
Online Access:	http://hdl.handle.net/10261/347361
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