A mutant O-GlcNAcase as a probe to reveal global dynamics of protein O-GlcNAcylation during Drosophila embryonic development
O-GlcNAcylation is a reversible type of serine/threonine glycosylation on nucleocytoplasmic proteins in metazoa. Various genetic approaches in several animal models have revealed that O-GlcNAcylation is essential for embryogenesis. However, the dynamic changes in global O-GlcNAcylation and the underlying mechanistic biology linking them to embryonic development is not understood. One of the limiting factors towards characterizing changes in O-GlcNAcylation has been the limited specificity of currently available tools to detect this modification. In the present study, harnessing the unusual properties of an O-GlcNAcase (OGA) mutant that binds O-GlcNAc (O-N-acetylglucosamine) sites with nanomolar affinity, we uncover changes in protein O-GlcNAcylation as a function of Drosophila development.
Main Authors: | , , , , , , , |
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Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Portland Press
2015-08-20
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Subjects: | O-GlcNAcase (OGA), Fluorescence polarization, Drosophila, embryonic development, O-GlcNAc probe, |
Online Access: | http://hdl.handle.net/10261/347361 |
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