Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential
This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO3 and leaf extract of Crataegus monogyna as the precursor material. In order to maximize the antibacterial efficacy against Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa, the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO3, pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution. The results showed that 20 mL of plant extract, 80 mL of AgNO3, pH 08, 100 °C temperature were the optimum reaction conditions under which we obtained the smallest Ag-NPs (7 nm). The scanning electron microscopy and X-ray diffraction analysis confirmed the spherical and crystalline nature of Ag-NPs. The antibacterial activity assay demonstrated a high antibacterial effect of Ag-NPs against S. aureus, P. mirabilis, K. pneumoniae and P. aeruginosa, and that impact was greater with smaller-sized nanoparticles (7 nm). This study shows that leaf extract of C. monogyna is a possible medium for the green fabrication of Ag-NPs, and control over reaction factors can establish the characteristics and antibacterial effectiveness of Ag-NPs.
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Format: | artículo biblioteca |
Language: | English |
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Taylor & Francis
2023-10-04
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Subjects: | Green synthesis, Crataegus monogyna, reaction parameters, antibacterial activity, |
Online Access: | http://hdl.handle.net/10261/341923 |
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dig-ipna-es-10261-3419232024-01-10T02:47:26Z Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential Mehmood, Ansar Zahir, Sobia Khan, Muhammad Abdul Rauf Ahmad, Khawaja Shafique Abasi, Fozia Raffi, Muhammad Proćków, Jarosław Pérez de Lastra, José Manuel Caja Canarias Green synthesis Crataegus monogyna reaction parameters antibacterial activity This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO3 and leaf extract of Crataegus monogyna as the precursor material. In order to maximize the antibacterial efficacy against Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa, the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO3, pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution. The results showed that 20 mL of plant extract, 80 mL of AgNO3, pH 08, 100 °C temperature were the optimum reaction conditions under which we obtained the smallest Ag-NPs (7 nm). The scanning electron microscopy and X-ray diffraction analysis confirmed the spherical and crystalline nature of Ag-NPs. The antibacterial activity assay demonstrated a high antibacterial effect of Ag-NPs against S. aureus, P. mirabilis, K. pneumoniae and P. aeruginosa, and that impact was greater with smaller-sized nanoparticles (7 nm). This study shows that leaf extract of C. monogyna is a possible medium for the green fabrication of Ag-NPs, and control over reaction factors can establish the characteristics and antibacterial effectiveness of Ag-NPs. This research was funded by Caja Canarias, Project 2019SP43. Peer reviewed 2024-01-09T11:20:57Z 2024-01-09T11:20:57Z 2023-10-04 artículo http://purl.org/coar/resource_type/c_6501 Journal of Biomolecular Structure and Dynamics: 1-11 (2023) 0739-1102 http://hdl.handle.net/10261/341923 10.1080/07391102.2023.2242960 1538-0254 en Publisher's version https://doi.org/10.1080/07391102.2023.2242960 Sí open Taylor & Francis |
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Green synthesis Crataegus monogyna reaction parameters antibacterial activity Green synthesis Crataegus monogyna reaction parameters antibacterial activity |
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Green synthesis Crataegus monogyna reaction parameters antibacterial activity Green synthesis Crataegus monogyna reaction parameters antibacterial activity Mehmood, Ansar Zahir, Sobia Khan, Muhammad Abdul Rauf Ahmad, Khawaja Shafique Abasi, Fozia Raffi, Muhammad Proćków, Jarosław Pérez de Lastra, José Manuel Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential |
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This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO3 and leaf extract of Crataegus monogyna as the precursor material. In order to maximize the antibacterial efficacy against Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa, the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO3, pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution. The results showed that 20 mL of plant extract, 80 mL of AgNO3, pH 08, 100 °C temperature were the optimum reaction conditions under which we obtained the smallest Ag-NPs (7 nm). The scanning electron microscopy and X-ray diffraction analysis confirmed the spherical and crystalline nature of Ag-NPs. The antibacterial activity assay demonstrated a high antibacterial effect of Ag-NPs against S. aureus, P. mirabilis, K. pneumoniae and P. aeruginosa, and that impact was greater with smaller-sized nanoparticles (7 nm). This study shows that leaf extract of C. monogyna is a possible medium for the green fabrication of Ag-NPs, and control over reaction factors can establish the characteristics and antibacterial effectiveness of Ag-NPs. |
author2 |
Caja Canarias |
author_facet |
Caja Canarias Mehmood, Ansar Zahir, Sobia Khan, Muhammad Abdul Rauf Ahmad, Khawaja Shafique Abasi, Fozia Raffi, Muhammad Proćków, Jarosław Pérez de Lastra, José Manuel |
format |
artículo |
topic_facet |
Green synthesis Crataegus monogyna reaction parameters antibacterial activity |
author |
Mehmood, Ansar Zahir, Sobia Khan, Muhammad Abdul Rauf Ahmad, Khawaja Shafique Abasi, Fozia Raffi, Muhammad Proćków, Jarosław Pérez de Lastra, José Manuel |
author_sort |
Mehmood, Ansar |
title |
Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential |
title_short |
Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential |
title_full |
Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential |
title_fullStr |
Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential |
title_full_unstemmed |
Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential |
title_sort |
optimization and bio-fabrication of phyto-mediated silver nanoparticles (ag-nps) for antibacterial potential |
publisher |
Taylor & Francis |
publishDate |
2023-10-04 |
url |
http://hdl.handle.net/10261/341923 |
work_keys_str_mv |
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