Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential

This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO3 and leaf extract of Crataegus monogyna as the precursor material. In order to maximize the antibacterial efficacy against Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa, the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO3, pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution. The results showed that 20 mL of plant extract, 80 mL of AgNO3, pH 08, 100 °C temperature were the optimum reaction conditions under which we obtained the smallest Ag-NPs (7 nm). The scanning electron microscopy and X-ray diffraction analysis confirmed the spherical and crystalline nature of Ag-NPs. The antibacterial activity assay demonstrated a high antibacterial effect of Ag-NPs against S. aureus, P. mirabilis, K. pneumoniae and P. aeruginosa, and that impact was greater with smaller-sized nanoparticles (7 nm). This study shows that leaf extract of C. monogyna is a possible medium for the green fabrication of Ag-NPs, and control over reaction factors can establish the characteristics and antibacterial effectiveness of Ag-NPs.

Saved in:
Bibliographic Details
Main Authors: Mehmood, Ansar, Zahir, Sobia, Khan, Muhammad Abdul Rauf, Ahmad, Khawaja Shafique, Abasi, Fozia, Raffi, Muhammad, Proćków, Jarosław, Pérez de Lastra, José Manuel
Other Authors: Caja Canarias
Format: artículo biblioteca
Language:English
Published: Taylor & Francis 2023-10-04
Subjects:Green synthesis, Crataegus monogyna, reaction parameters, antibacterial activity,
Online Access:http://hdl.handle.net/10261/341923
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-ipna-es-10261-341923
record_format koha
spelling dig-ipna-es-10261-3419232024-01-10T02:47:26Z Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential Mehmood, Ansar Zahir, Sobia Khan, Muhammad Abdul Rauf Ahmad, Khawaja Shafique Abasi, Fozia Raffi, Muhammad Proćków, Jarosław Pérez de Lastra, José Manuel Caja Canarias Green synthesis Crataegus monogyna reaction parameters antibacterial activity This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO3 and leaf extract of Crataegus monogyna as the precursor material. In order to maximize the antibacterial efficacy against Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa, the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO3, pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution. The results showed that 20 mL of plant extract, 80 mL of AgNO3, pH 08, 100 °C temperature were the optimum reaction conditions under which we obtained the smallest Ag-NPs (7 nm). The scanning electron microscopy and X-ray diffraction analysis confirmed the spherical and crystalline nature of Ag-NPs. The antibacterial activity assay demonstrated a high antibacterial effect of Ag-NPs against S. aureus, P. mirabilis, K. pneumoniae and P. aeruginosa, and that impact was greater with smaller-sized nanoparticles (7 nm). This study shows that leaf extract of C. monogyna is a possible medium for the green fabrication of Ag-NPs, and control over reaction factors can establish the characteristics and antibacterial effectiveness of Ag-NPs. This research was funded by Caja Canarias, Project 2019SP43. Peer reviewed 2024-01-09T11:20:57Z 2024-01-09T11:20:57Z 2023-10-04 artículo http://purl.org/coar/resource_type/c_6501 Journal of Biomolecular Structure and Dynamics: 1-11 (2023) 0739-1102 http://hdl.handle.net/10261/341923 10.1080/07391102.2023.2242960 1538-0254 en Publisher's version https://doi.org/10.1080/07391102.2023.2242960 Sí open Taylor & Francis
institution IPNA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ipna-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IPNA España
language English
topic Green synthesis
Crataegus monogyna
reaction parameters
antibacterial activity
Green synthesis
Crataegus monogyna
reaction parameters
antibacterial activity
spellingShingle Green synthesis
Crataegus monogyna
reaction parameters
antibacterial activity
Green synthesis
Crataegus monogyna
reaction parameters
antibacterial activity
Mehmood, Ansar
Zahir, Sobia
Khan, Muhammad Abdul Rauf
Ahmad, Khawaja Shafique
Abasi, Fozia
Raffi, Muhammad
Proćków, Jarosław
Pérez de Lastra, José Manuel
Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential
description This report examines the bio-fabrication of silver nanoparticles (Ag-NPs) utilizing AgNO3 and leaf extract of Crataegus monogyna as the precursor material. In order to maximize the antibacterial efficacy against Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumoniae and Pseudomonas aeruginosa, the reaction conditions for the green fabrication of Ag-NPs were optimized. A one factor at a time approach (volume concentration of extract, volume concentration of AgNO3, pH and temperature) was used to optimize the best condition, and results were assessed through UV-visible spectroscopy and particle size distribution. The results showed that 20 mL of plant extract, 80 mL of AgNO3, pH 08, 100 °C temperature were the optimum reaction conditions under which we obtained the smallest Ag-NPs (7 nm). The scanning electron microscopy and X-ray diffraction analysis confirmed the spherical and crystalline nature of Ag-NPs. The antibacterial activity assay demonstrated a high antibacterial effect of Ag-NPs against S. aureus, P. mirabilis, K. pneumoniae and P. aeruginosa, and that impact was greater with smaller-sized nanoparticles (7 nm). This study shows that leaf extract of C. monogyna is a possible medium for the green fabrication of Ag-NPs, and control over reaction factors can establish the characteristics and antibacterial effectiveness of Ag-NPs.
author2 Caja Canarias
author_facet Caja Canarias
Mehmood, Ansar
Zahir, Sobia
Khan, Muhammad Abdul Rauf
Ahmad, Khawaja Shafique
Abasi, Fozia
Raffi, Muhammad
Proćków, Jarosław
Pérez de Lastra, José Manuel
format artículo
topic_facet Green synthesis
Crataegus monogyna
reaction parameters
antibacterial activity
author Mehmood, Ansar
Zahir, Sobia
Khan, Muhammad Abdul Rauf
Ahmad, Khawaja Shafique
Abasi, Fozia
Raffi, Muhammad
Proćków, Jarosław
Pérez de Lastra, José Manuel
author_sort Mehmood, Ansar
title Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential
title_short Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential
title_full Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential
title_fullStr Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential
title_full_unstemmed Optimization and bio-fabrication of phyto-mediated silver nanoparticles (Ag-NPs) for antibacterial potential
title_sort optimization and bio-fabrication of phyto-mediated silver nanoparticles (ag-nps) for antibacterial potential
publisher Taylor & Francis
publishDate 2023-10-04
url http://hdl.handle.net/10261/341923
work_keys_str_mv AT mehmoodansar optimizationandbiofabricationofphytomediatedsilvernanoparticlesagnpsforantibacterialpotential
AT zahirsobia optimizationandbiofabricationofphytomediatedsilvernanoparticlesagnpsforantibacterialpotential
AT khanmuhammadabdulrauf optimizationandbiofabricationofphytomediatedsilvernanoparticlesagnpsforantibacterialpotential
AT ahmadkhawajashafique optimizationandbiofabricationofphytomediatedsilvernanoparticlesagnpsforantibacterialpotential
AT abasifozia optimizationandbiofabricationofphytomediatedsilvernanoparticlesagnpsforantibacterialpotential
AT raffimuhammad optimizationandbiofabricationofphytomediatedsilvernanoparticlesagnpsforantibacterialpotential
AT prockowjarosław optimizationandbiofabricationofphytomediatedsilvernanoparticlesagnpsforantibacterialpotential
AT perezdelastrajosemanuel optimizationandbiofabricationofphytomediatedsilvernanoparticlesagnpsforantibacterialpotential
_version_ 1792484815396667392