Reduced Mid1 expression and delayed neuromotor development in daDREAM transgenic mice

Downstream regulatory element antagonist modulator (DREAM) is a C a2+-binding protein that binds DNA and represses transcription in a Ca 2+-dependent manner. Previous work has shown a role for DREAM in cerebellar function regulating the expression of the sodium/calcium exchanger 3 (NCX3) in cerebellar granular neurons to control Ca 2+ homeostasis and survival of these neurons. To achieve a global view of the genes regulated by DREAM in the cerebellum, we performed a genome-wide analysis in transgenic cerebellum expressing a Ca 2+-insensitive/CREB-independent dominant active mutant DREAM (daDREAM). Here we show that DREAM regulates the expression of the midline 1 (Mid1) gene early after birth. As a consequence, daDREAM mice exhibit a significant shortening of the rostro-caudal axis of the cerebellum and a delay in neuromotor development early after birth. Our results indicate a role for DREAM in cerebellar function. © 2012 Dierssen, Fedrizzi, Gomez-Villafuertes, de Lagran, Gutierrez-Adan, Sahún, Pintado, Oliveros, Dopazo, Gonzalez, Brini, Mellström, Carafoli and Naranjo.

Bibliographic Details

Main Authors:	Dierssen, M., Fedrizzi, L., Gomez-Villafuertes, R., de Lagran, M. M., Gutierrez-Adan, A., Sahún, I., Pintado, B., Oliveros, J. C., Dopazo, X. M., Gonzalez, P., Brini, M., Mellström, B., Carafoli, E., Naranjo, J. R.
Format:	journal article biblioteca
Language:	eng
Published:	2012
Online Access:	http://hdl.handle.net/20.500.12792/5043
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