Reduced susceptibility to bovine spongiform encephalopathy prions in transgenic mice expressing a bovine PrP with five octapeptide repeats

In this work, transgenic (Tg) mice were generated expressing a bovine prion protein containing five octarepeats (BoPrP5OR-Tg). After intracerebral inoculation of bovine spongiform encephalopathy (BSE) inoculum, these mice suffered a BSE-like neuropathology but survived longer compared with homologous Tg mice expressing similar levels of a six octarepeat BoPrP protein (BoPrp6OR-Tg). De novo-generated five octarepeat (50R) PrPSc showed no biochemical differences from 60R-PrPsc, and the proteinase K-resistant core (Prpres) was biochemically indistinguishable from the 60R counterpart. Lower susceptibility to BSE is suggested for BoPrp5OR-Tg mice, as they were not as efficient at replicating BSE prions from the same natural source inoculum as BoPrp60R-Tg mice expressing similar PrPC levels. These results raise the possibility of selecting cattle breeds bearing the 50R Prnp allele that are less susceptible to prion infection. © 2007 SGM.

Bibliographic Details

Main Authors:	Brun, A., Gutiérrez-Adán, A., Castilla, J., Pintado, B., Díaz-San Segundo, F., Cano, M. J., Alamillo, E., Espinosa, J. C., Torres, J. M.
Format:	journal article biblioteca
Language:	eng
Published:	2007
Online Access:	http://hdl.handle.net/20.500.12792/4663
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