Giant panda (Ailuropoda melanoleuca) sperm morphometry and function after repeated freezing and thawing
This work examines the effects of subsequent cycles of freezing-thawing on giant panda (Ailuropoda melanoleuca) sperm morphometry and function, and assesses whether density-gradient centrifugation (DGC) can increase the number of freezing-thawing cycles this sperm can withstand. A sperm sample was collected by electroejaculation from a mature giant panda and subjected to five freezing-thawing cycles. Although repeated freezing-thawing negatively affected (P < 0.05) sperm motility and membrane integrity, in both nonselected and DCG-selected sperm samples, >60% of the sperm cells in both treatments showed acrosome integrity even after the fifth freezing cycle. In fresh semen, the sperm head length was 4.7 μm, the head width 3.6 μm, area 14.3 μm2 and perimeter length 14.1 μm. The present results suggest that giant panda sperm trends to be resistant to repeated freezing-thawing, even without DGC selection. © 2016 Blackwell Verlag GmbH.
| Main Authors: | , , , , , , , , , , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
2016
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| Online Access: | http://hdl.handle.net/20.500.12792/4176 |
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