Characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin
Fourteen monoclonal antibodies which recognise antigenic determinants on heavy and light chains of trout immunoglobulins have been characterised. Experiments on immunoprecipitation and immunostaining of lymphoid cells carried out with these monoclonal antibodies suggest the existence of antigenic variants within trout immunoglobulin. Most of these variants seem to be isotypes, since they were present in each of 30 sera from individual trout tested. Analysis of the immunoprecipitates by SDS-PAGE showed two light chains of different molecular weight. Cross reactivity patterns of these monoclonal antibodies with sera from four species and one subspecies of salmonid indicate a high conservation of the epitopes recognised, although four monoclonal antibodies show a more restricted reactivity. © 1993 Academic Press. All rights reserved.
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
1993
|
| Online Access: | http://hdl.handle.net/20.500.12792/4019 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-inia-es-20.500.12792-4019 |
|---|---|
| record_format |
koha |
| spelling |
dig-inia-es-20.500.12792-40192020-12-15T09:54:33Z Characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin Sánchez, C. López-Fierro, P. Zapata, A. Dominguez, J. Fourteen monoclonal antibodies which recognise antigenic determinants on heavy and light chains of trout immunoglobulins have been characterised. Experiments on immunoprecipitation and immunostaining of lymphoid cells carried out with these monoclonal antibodies suggest the existence of antigenic variants within trout immunoglobulin. Most of these variants seem to be isotypes, since they were present in each of 30 sera from individual trout tested. Analysis of the immunoprecipitates by SDS-PAGE showed two light chains of different molecular weight. Cross reactivity patterns of these monoclonal antibodies with sera from four species and one subspecies of salmonid indicate a high conservation of the epitopes recognised, although four monoclonal antibodies show a more restricted reactivity. © 1993 Academic Press. All rights reserved. 2020-10-22T16:00:34Z 2020-10-22T16:00:34Z 1993 journal article http://hdl.handle.net/20.500.12792/4019 10.1006/fsim.1993.1024 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
| institution |
INIA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-inia-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del INIA España |
| language |
eng |
| description |
Fourteen monoclonal antibodies which recognise antigenic determinants on heavy and light chains of trout immunoglobulins have been characterised. Experiments on immunoprecipitation and immunostaining of lymphoid cells carried out with these monoclonal antibodies suggest the existence of antigenic variants within trout immunoglobulin. Most of these variants seem to be isotypes, since they were present in each of 30 sera from individual trout tested. Analysis of the immunoprecipitates by SDS-PAGE showed two light chains of different molecular weight. Cross reactivity patterns of these monoclonal antibodies with sera from four species and one subspecies of salmonid indicate a high conservation of the epitopes recognised, although four monoclonal antibodies show a more restricted reactivity. © 1993 Academic Press. All rights reserved. |
| format |
journal article |
| author |
Sánchez, C. López-Fierro, P. Zapata, A. Dominguez, J. |
| spellingShingle |
Sánchez, C. López-Fierro, P. Zapata, A. Dominguez, J. Characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin |
| author_facet |
Sánchez, C. López-Fierro, P. Zapata, A. Dominguez, J. |
| author_sort |
Sánchez, C. |
| title |
Characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin |
| title_short |
Characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin |
| title_full |
Characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin |
| title_fullStr |
Characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin |
| title_full_unstemmed |
Characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin |
| title_sort |
characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin |
| publishDate |
1993 |
| url |
http://hdl.handle.net/20.500.12792/4019 |
| work_keys_str_mv |
AT sanchezc characterisationofmonoclonalantibodiesagainstheavyandlightchainsoftroutimmunoglobulin AT lopezfierrop characterisationofmonoclonalantibodiesagainstheavyandlightchainsoftroutimmunoglobulin AT zapataa characterisationofmonoclonalantibodiesagainstheavyandlightchainsoftroutimmunoglobulin AT dominguezj characterisationofmonoclonalantibodiesagainstheavyandlightchainsoftroutimmunoglobulin |
| _version_ |
1758004540903260160 |