Characterisation of monoclonal antibodies against heavy and light chains of trout immunoglobulin
Fourteen monoclonal antibodies which recognise antigenic determinants on heavy and light chains of trout immunoglobulins have been characterised. Experiments on immunoprecipitation and immunostaining of lymphoid cells carried out with these monoclonal antibodies suggest the existence of antigenic variants within trout immunoglobulin. Most of these variants seem to be isotypes, since they were present in each of 30 sera from individual trout tested. Analysis of the immunoprecipitates by SDS-PAGE showed two light chains of different molecular weight. Cross reactivity patterns of these monoclonal antibodies with sera from four species and one subspecies of salmonid indicate a high conservation of the epitopes recognised, although four monoclonal antibodies show a more restricted reactivity. © 1993 Academic Press. All rights reserved.
| Main Authors: | , , , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
1993
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| Online Access: | http://hdl.handle.net/20.500.12792/4019 |
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