Characterization of potato potyvirus Y (PVY) isolates from seed potato batches. Situation of the NTN, Wilga and Z isolates
A collection of 38 PVY isolates from seed potato batches, originating from several Western European countries, was characterized by using current biological, serological and molecular tools differentiating PVY strains and groups. The correlation between the three kinds of tests was good but not absolute. No single serological or PCR method was able to discriminate among the five isolate groups found. Twenty-nine isolates belonged to the PVY(N) strain and six to the PVY(O) strain. No PVY(C) was found. Two other isolates reacted serologically like PVY(O), but were unable to elicit a hypersensitive response from the Ny(tbr) gene and probably represent the PVY(Z) group. At the molecular level, these two isolates showed a combination of both PVY(O) and PVY(N) and could be recombinants of these strains. Another isolate reacted serologically like PVY(O), but induced vein necrosis in tobacco, like PVY(N)-Wilga. Some PVY(N) isolates caused tuber ring necrosis in glasshouse conditions. These might belong to the PVY(NTN) group. The PVY(NTN), PVY(N)-Wilga and PVY(Z) groups probably represent pathotypes within strains PVY(N) and PVY(O), respectively. The present study also confirms previous reports showing a high genetic variation at the 5' end within the PVY(N) strain.
| Main Authors: | , , , , , , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
1998
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| Online Access: | http://hdl.handle.net/20.500.12792/3896 |
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