Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley
EPSPS (5-enolpyruvilshikimate-3-phosphate syn-thase) inhibition has been described as the main target for the herbicide glyphosate. Total soluble protein concentration and EPSPS activity were measured after 4 hr, 1 day and 7 day of glyphosate treatment, at a dose equivalent to 0.94 Kg ha-1. After seven days of glyphosate treatment, EPSPS activity reduced to 80% as compared to the activity in untreated seedlings. Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM, but inhibition increased to 64% when PEP concentration was 0.1 mM. Moreover, 1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP, instead 0.1 mM of glyphosate was necessary when 1 mM was present in the reactions.
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| Format: | journal article biblioteca |
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1996
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| Online Access: | http://hdl.handle.net/20.500.12792/3679 |
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dig-inia-es-20.500.12792-36792020-12-15T09:54:31Z Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley Rebordinos, L. Escorial, C. Garcia-Baudin, J. M. Chueca, M. C. EPSPS (5-enolpyruvilshikimate-3-phosphate syn-thase) inhibition has been described as the main target for the herbicide glyphosate. Total soluble protein concentration and EPSPS activity were measured after 4 hr, 1 day and 7 day of glyphosate treatment, at a dose equivalent to 0.94 Kg ha-1. After seven days of glyphosate treatment, EPSPS activity reduced to 80% as compared to the activity in untreated seedlings. Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM, but inhibition increased to 64% when PEP concentration was 0.1 mM. Moreover, 1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP, instead 0.1 mM of glyphosate was necessary when 1 mM was present in the reactions. 2020-10-22T15:12:30Z 2020-10-22T15:12:30Z 1996 journal article http://hdl.handle.net/20.500.12792/3679 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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España |
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ES |
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dig-inia-es |
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biblioteca |
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Europa del Sur |
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Biblioteca del INIA España |
| language |
eng |
| description |
EPSPS (5-enolpyruvilshikimate-3-phosphate syn-thase) inhibition has been described as the main target for the herbicide glyphosate. Total soluble protein concentration and EPSPS activity were measured after 4 hr, 1 day and 7 day of glyphosate treatment, at a dose equivalent to 0.94 Kg ha-1. After seven days of glyphosate treatment, EPSPS activity reduced to 80% as compared to the activity in untreated seedlings. Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM, but inhibition increased to 64% when PEP concentration was 0.1 mM. Moreover, 1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP, instead 0.1 mM of glyphosate was necessary when 1 mM was present in the reactions. |
| format |
journal article |
| author |
Rebordinos, L. Escorial, C. Garcia-Baudin, J. M. Chueca, M. C. |
| spellingShingle |
Rebordinos, L. Escorial, C. Garcia-Baudin, J. M. Chueca, M. C. Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley |
| author_facet |
Rebordinos, L. Escorial, C. Garcia-Baudin, J. M. Chueca, M. C. |
| author_sort |
Rebordinos, L. |
| title |
Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley |
| title_short |
Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley |
| title_full |
Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley |
| title_fullStr |
Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley |
| title_full_unstemmed |
Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley |
| title_sort |
activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley |
| publishDate |
1996 |
| url |
http://hdl.handle.net/20.500.12792/3679 |
| work_keys_str_mv |
AT rebordinosl activityof5enolpyruvylshikimate3phosphatesynthaseinglyphosatetreatedanduntreatedbarley AT escorialc activityof5enolpyruvylshikimate3phosphatesynthaseinglyphosatetreatedanduntreatedbarley AT garciabaudinjm activityof5enolpyruvylshikimate3phosphatesynthaseinglyphosatetreatedanduntreatedbarley AT chuecamc activityof5enolpyruvylshikimate3phosphatesynthaseinglyphosatetreatedanduntreatedbarley |
| _version_ |
1758004781935230976 |