Activity of 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate treated and untreated barley
EPSPS (5-enolpyruvilshikimate-3-phosphate syn-thase) inhibition has been described as the main target for the herbicide glyphosate. Total soluble protein concentration and EPSPS activity were measured after 4 hr, 1 day and 7 day of glyphosate treatment, at a dose equivalent to 0.94 Kg ha-1. After seven days of glyphosate treatment, EPSPS activity reduced to 80% as compared to the activity in untreated seedlings. Glyphosate at 2 μM concentration inhibited EPSPS by 18% at a PEP concentration of 1 mM, but inhibition increased to 64% when PEP concentration was 0.1 mM. Moreover, 1.5 μM glyphosate concentration reduced EPSPS activity by 50% at 0.1 mM of PEP, instead 0.1 mM of glyphosate was necessary when 1 mM was present in the reactions.
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| Main Authors: | , , , |
|---|---|
| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
1996
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| Online Access: | http://hdl.handle.net/20.500.12792/3679 |
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