Effect of reduced concentrations of glycerol and various macromolecules on the cryopreservation of mouse and cattle embryos
The effect of different macromolecules [bovine serum albumin (BSA), Pluronic F-68, (ET surfactant), or sodium hyaluronate (SH)] on postthaw survival of mouse morulae and in vivo- and in vitro-derived bovine blastocysts frozen in 10, 5, or 1% glycerol solutions was investigated. Embryos were equilibrated with cryoprotectant solution at 25°C for 10 min, seeded at -5°C, cooled at 0.5°C/min to -35°C, and plunged into liquid nitrogen. Embryos were thawed in a 35°C water bath, glycerol was removed with 0.6 M sucrose at 25°C for 5 min, and postthaw viability was evaluated after 1, 24, and 48 h in culture. The addition of BSA supplementation improved postthaw survival of mouse morulae frozen in 5% glycerol, but not in 10% glycerol. All three macromolecular supplements were effective in increasing survival of mouse morulae in 5% glycerol but only BSA and SH were effective in increasing postthaw survival of in vivo- and in vitro-derived bovine blastocysts. None of the macromolecular supplements improved postthaw survival of embryos frozen in 1% glycerol. (C) 2000 Academic Press.
Main Authors: | , , , |
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Format: | journal article biblioteca |
Language: | eng |
Published: |
2000
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Online Access: | http://hdl.handle.net/20.500.12792/3293 |
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