First report on the detection and quantification of Verticillium dahliae from Estonian strawberry fields using quantitative real-time PCR
The main aim of this investigation was to develop a SYBRGreen real-time polymerase chain reaction (PCR) assay for detection and quantification of Verticillium dahliae directly from affected strawberry roots and soils. The proposed assay utilizes a specifically designed primer pair on the basis of an internal transcribed spacer (ITS) sequence. During 2014-2015, plant and soil samples were randomly collected from different areas including Vasula, Rohu, Unipiha, Utsu and Marjamaa in Estonia and analyzed for V. dahliae. Real-time PCR technique using primers designed to the rDNA ITS1 was highly sensitive and accurate and so allowed reliable quantification of the pathogen DNA at low inoculation in soils (3 × 10-1 pg µl-1) and even in root of symptomless plants (5 × 10-1 pg µl-1). This is the first study of using this technique to quantify the population of V. dahliae in strawberry fields from Estonia. © 2016, Lithuanian Institute of Agriculture. All rights reserved.
| Main Authors: | , , , |
|---|---|
| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
2016
|
| Online Access: | http://hdl.handle.net/20.500.12792/2912 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|