Construction and isolation of recombinant vaccinia virus using genetic markers
The standard approach for the isolation of vaccinia virus recombinants involves homologous recombination between a transfected plasmid and the replicating viral DNA. In a typical infection/transfection experiment, recombinant viruses only account for a tiny proportion (10-4 to 10-3) of the progeny virus; thus, genetic markers are often included in the transfected plasmid to facilitate the selection of recombinant viruses. This chapter describes in detail two different selection procedures one relies on plaque formation phenotype using the vaccinia virus gene F13L; the other relies on antibiotic resistance using the Escherichia coli xanthine-guanine phosphoribosyl transferase gene.
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| Main Authors: | , , |
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| Format: | review biblioteca |
| Language: | eng |
| Published: |
2004
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| Online Access: | http://hdl.handle.net/20.500.12792/2506 |
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