Oral immunogenicity of the plant derived spike protein from swine-transmissible gastroenteritis coronavirus
Transgenic plants represent an inexpensive alternative to classical fermentation systems for production of recombinant subunit vaccines. Transgenic potato plants were created that express the N-terminal domain of the glycoprotein S (N-gS) from Transmissible gastroenteritis coronavirus (TGEV), containing the major antigenic sites of the protein. Extracts from potato tubers expressing N-gS were inoculated intraperitoneally to mice, and the vaccinated mice developed serum IgG specific for TGEV. Furthermore, when potato tubers expressing N-gS were fed directly to mice, they developed serum antibodies specific for gS protein, demonstrating the oral immunogenicity of the plant derived spike protein from TGEV.
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| Main Authors: | , , , , , , |
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| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
2000
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| Online Access: | http://hdl.handle.net/20.500.12792/1778 |
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