An inhibitor of polyprotein processing with the characteristics of a natural virus resistance factor.
Seedlings of Vigna unguiculata cv. Arlington are immune to cowpea mosaic virus (CPMV) but susceptible to cowpea severe mosaic virus (CPSMV). The CPMV resistance was controlled by a single dominant gene in crosses to the susceptible line Blackeye 5. Partially fractionated extracts of leaves of Arlington showed inhibitory activity against the proteolytic processing of a CPMV polyprotein. The leaf extracts also showed 2 other activities to a greater degree than did Blackeye 5: they inhibited the translation of CPMV RNAs and contained proteinases that degraded CPMV-encoded proteins. The proteinases degraded CPSMV and CPMV proteins equally well, however, and were not coinherited with CPMV immunity. The inhibitor of CPMV polyprotein processing possessed CPMV specificty and was coinherited with immunity, suggesting that it is the agent conveying immunity to CPMV. The inheritance of the translation inhibitor activities was complex and results suggested that one or more of these activities may contribute to CPMV immunity.
| Main Authors: | , , |
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| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
American Phytopathological Society
1988-01-05
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| Online Access: | http://hdl.handle.net/10261/364513 |
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