Effect of bta-miR-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and signalling pathways related to proliferation and differentiation in early embryo development through post-transcriptional mechanisms. A previous study showed that bta-mir 483-3p was exclusively detected in extracellular vesicles from the oviductal fluid of pregnant cows compared to non-pregnant (Mazzarella et al., 2021). Therefore, we aimed to determine whether bta-miR-483-3p is uptaken by bovine in vitro-produced embryos through passive transfection (gymnosis) and to evaluate the effect of miRNA supplementation on early embryo development and quality.
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2023-05-03
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dig-inia-es-10261-3514102024-03-21T14:29:44Z Effect of bta-miR-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development Mazzarella, Rosane Cajas, Yulia N. Cañón-Beltrán, Karina Gascón Collado, D. Beltrán Breña, Paula Sánchez, José María Fernández-Fuertes, Beatriz Gutiérrez-Adán, Alfonso González, E. Rizos, Dimitrios Mazzarella, Rosane [0000-0001-6860-8897] Cajas, Yulia N. [0000-0001-9791-6733] Cañón-Beltrán, Karina [0000-0002-0279-0857] Sánchez, José María [0000-0003-3889-2342] Fernández-Fuertes, Beatriz [0000-0003-2303-8733] Gutiérrez-Adán, Alfonso [0000-0001-9893-9179] Rizos, Dimitrios [0000-0001-6813-3940] MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and signalling pathways related to proliferation and differentiation in early embryo development through post-transcriptional mechanisms. A previous study showed that bta-mir 483-3p was exclusively detected in extracellular vesicles from the oviductal fluid of pregnant cows compared to non-pregnant (Mazzarella et al., 2021). Therefore, we aimed to determine whether bta-miR-483-3p is uptaken by bovine in vitro-produced embryos through passive transfection (gymnosis) and to evaluate the effect of miRNA supplementation on early embryo development and quality. Peer reviewed 2024-03-21T14:29:44Z 2024-03-21T14:29:44Z 2023-05-03 comunicación de congreso Animal - Science proceedings 14(3): 467-468 (2023) 2772-283X http://hdl.handle.net/10261/351410 10.1016/j.anscip.2023.03.060 en Departamento de Reproducción Animal Publisher's version https://doi.org/10.1016/j.anscip.2023.03.060 Sí open Elsevier |
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MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and signalling pathways related to proliferation and differentiation in early embryo development through post-transcriptional mechanisms. A previous study showed that bta-mir 483-3p was exclusively detected in extracellular vesicles from the oviductal fluid of pregnant cows compared to non-pregnant (Mazzarella et al., 2021). Therefore, we aimed to determine whether bta-miR-483-3p is uptaken by bovine in vitro-produced embryos through passive
transfection (gymnosis) and to evaluate the effect of miRNA supplementation on early embryo development and quality. |
author2 |
Mazzarella, Rosane [0000-0001-6860-8897] |
author_facet |
Mazzarella, Rosane [0000-0001-6860-8897] Mazzarella, Rosane Cajas, Yulia N. Cañón-Beltrán, Karina Gascón Collado, D. Beltrán Breña, Paula Sánchez, José María Fernández-Fuertes, Beatriz Gutiérrez-Adán, Alfonso González, E. Rizos, Dimitrios |
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comunicación de congreso |
author |
Mazzarella, Rosane Cajas, Yulia N. Cañón-Beltrán, Karina Gascón Collado, D. Beltrán Breña, Paula Sánchez, José María Fernández-Fuertes, Beatriz Gutiérrez-Adán, Alfonso González, E. Rizos, Dimitrios |
spellingShingle |
Mazzarella, Rosane Cajas, Yulia N. Cañón-Beltrán, Karina Gascón Collado, D. Beltrán Breña, Paula Sánchez, José María Fernández-Fuertes, Beatriz Gutiérrez-Adán, Alfonso González, E. Rizos, Dimitrios Effect of bta-miR-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development |
author_sort |
Mazzarella, Rosane |
title |
Effect of bta-miR-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development |
title_short |
Effect of bta-miR-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development |
title_full |
Effect of bta-miR-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development |
title_fullStr |
Effect of bta-miR-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development |
title_full_unstemmed |
Effect of bta-miR-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development |
title_sort |
effect of bta-mir-483-3p of extracellular vesicles from the oviductal fluid of pregnant cows on in vitro early embryo development |
publisher |
Elsevier |
publishDate |
2023-05-03 |
url |
http://hdl.handle.net/10261/351410 |
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