Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type
8 Pág.
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2022-08-05
|
| Subjects: | Freezing, Germ cells, Sperm, Testicular tissue, Vitrification, Cryopreservation, |
| Online Access: | http://hdl.handle.net/10261/304712 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85135721944 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-inia-es-10261-304712 |
|---|---|
| record_format |
koha |
| spelling |
dig-inia-es-10261-3047122024-10-27T21:47:26Z Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type Picazo, C M. Castaño, Cristina Bóveda, Paula Toledano-Díaz, A. Velázquez, Rosario Pequeño, Belén Esteso, Milagros C. Gadea, J. Villaverde-Morcillo, S. Cerdeira, J. Santiago Moreno, Julián Agencia Estatal de Investigación (España) Comunidad de Madrid Centro de Recuperación de Animales Silvestres (España) Centro Veterinario Aluche-Las Águilas Castaño, C. [0000-0003-1134-1436] Bóveda, P. [0000-0001-5749-4345] Toledano Díaz, Adolfo [0000-0001-6679-485X] Esteso, Milagros C. [0000-0002-8963-5736] Gadea, J. [0000-0002-3474-7626] Villaverde-Morcillo, S. [0000-0002-4879-6007] Santiago Moreno, Julián [0000-0001-5551-8120] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Freezing Germ cells Sperm Testicular tissue Vitrification Cryopreservation 8 Pág. Sperm cryopreservation is the most common procedure used to establish germplasm banks for endangered species - but sometimes sperm cells cannot be obtained. In such cases, freezing testicular tissue may be the only option. The testes contains germ cells at different stages of differentiation, including spermatogonia, primary spermatocytes, secondary spermatocytes, spermatids, and spermatozoa, among which differences in cryoresistance might be expected. The present work compares the viability and DNA integrity of 'rounded' cells, and of elongated spermatids and spermatozoa, from the dog and wild boar, following the cryopreservation of testicular tissue by slow freezing or vitrification. Cell viability was analyzed by PI/SYBR14 staining, and DNA integrity via the TUNEL technique. For wild boar, no significant differences were seen between the two methods with respect to the percentage of viable cells, nor in the percentage of cells with DNA damage. In the dog, the percentage of viable rounded germ cells (65.0 ± 2.4%) was higher (P < 0.05) after vitrification than after slow freezing (45.1 ± 6.7%). No difference was found between the two methods in terms of the viability of elongated cells. For rounded cells, the percentage of intact DNA was greater (P < 0.05) after vitrification (90.5 ± 2.1%) than after slow freezing (42.6 ± 11.0%), while for elongated spermatids and spermatozoa it was higher (P < 0.05) after slow freezing (66.9 ± 6.1%) than after vitrification (50.7 ± 4.5%). Thus, the response to cryopreservation is cell type-, cryopreservation type-, and species-dependent. Vitrification would appear to be the most appropriate method for preserving dog testicular tissue given the associated high cell viability and low degree of DNA fragmentation, while in wild boar, either method might be used. This study was funded by grant PID2020-113288RB-100/AEI/10.13039/501100011033. Peer reviewed 2023-03-29T08:47:01Z 2023-03-29T08:47:01Z 2022-08-05 artículo http://purl.org/coar/resource_type/c_6501 Theriogenology 190: 65-72 (2022) 0093-691X http://hdl.handle.net/10261/304712 10.1016/j.theriogenology.2022.07.020 1879-3231 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011033 35963122 2-s2.0-85135721944 https://api.elsevier.com/content/abstract/scopus_id/85135721944 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113288RB-I00/ES/MECANISMOS DE CRIO-RESISTENCIA ESPERMATICA Y NUEVOS PROCEDIMIENTOS DE CRIOPRESERVACION ESPERMATICA Y TEJIDO TESTICULAR EN RUMIANTES DOMESTICOS Y SILVESTRES/ Departamento de Reproducción Animal Publisher's version https://doi.org/10.1016/j.theriogenology.2022.07.020 Sí open Elsevier |
| institution |
INIA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-inia-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del INIA España |
| language |
English |
| topic |
Freezing Germ cells Sperm Testicular tissue Vitrification Cryopreservation Freezing Germ cells Sperm Testicular tissue Vitrification Cryopreservation |
| spellingShingle |
Freezing Germ cells Sperm Testicular tissue Vitrification Cryopreservation Freezing Germ cells Sperm Testicular tissue Vitrification Cryopreservation Picazo, C M. Castaño, Cristina Bóveda, Paula Toledano-Díaz, A. Velázquez, Rosario Pequeño, Belén Esteso, Milagros C. Gadea, J. Villaverde-Morcillo, S. Cerdeira, J. Santiago Moreno, Julián Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type |
| description |
8 Pág. |
| author2 |
Agencia Estatal de Investigación (España) |
| author_facet |
Agencia Estatal de Investigación (España) Picazo, C M. Castaño, Cristina Bóveda, Paula Toledano-Díaz, A. Velázquez, Rosario Pequeño, Belén Esteso, Milagros C. Gadea, J. Villaverde-Morcillo, S. Cerdeira, J. Santiago Moreno, Julián |
| format |
artículo |
| topic_facet |
Freezing Germ cells Sperm Testicular tissue Vitrification Cryopreservation |
| author |
Picazo, C M. Castaño, Cristina Bóveda, Paula Toledano-Díaz, A. Velázquez, Rosario Pequeño, Belén Esteso, Milagros C. Gadea, J. Villaverde-Morcillo, S. Cerdeira, J. Santiago Moreno, Julián |
| author_sort |
Picazo, C M. |
| title |
Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type |
| title_short |
Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type |
| title_full |
Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type |
| title_fullStr |
Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type |
| title_full_unstemmed |
Cryopreservation of testicular tissue from the dog (Canis familiaris) and wild boar (Sus scrofa) by slow freezing and vitrification: Differences in cryoresistance according to cell type |
| title_sort |
cryopreservation of testicular tissue from the dog (canis familiaris) and wild boar (sus scrofa) by slow freezing and vitrification: differences in cryoresistance according to cell type |
| publisher |
Elsevier |
| publishDate |
2022-08-05 |
| url |
http://hdl.handle.net/10261/304712 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85135721944 |
| work_keys_str_mv |
AT picazocm cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT castanocristina cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT bovedapaula cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT toledanodiaza cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT velazquezrosario cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT pequenobelen cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT estesomilagrosc cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT gadeaj cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT villaverdemorcillos cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT cerdeiraj cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype AT santiagomorenojulian cryopreservationoftesticulartissuefromthedogcanisfamiliarisandwildboarsusscrofabyslowfreezingandvitrificationdifferencesincryoresistanceaccordingtocelltype |
| _version_ |
1816136364769411072 |