Methanol toxicity on the embrionary development of Oryzias latipes
In order to optimize the conditions on Medaka fish (Oryzias latipes) as in vitro assay to study embrionary development effects, a range of methanol dilutions were studied to know the maximum permissible without no observable effects. This solvent will be employed in the next future as a vehicle of organic concentrates in fraction samples methodologies. Assays with Petri dishes and vials were performed, in exposures on different dilutions of methanol to 1 and 1.5% and 0.5 and 1%, for Petri dishes and vials, respectively. Controls were performed in each case. Results obtained pointed out that in the dilutions performed in Petri dishes, no macroscopic lesions were observed at 1% even at 1.5% do it; however the emergence larval delayed more time than vials. Nevertheless the percentage of hatching in both of the cases, showed that the closed vials become more synchronized and advance the hatching. Results suggest that not more than 0.5% of methanol can be used if used as vehicle of organic compounds.
| Main Authors: | , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Asociación Española de Toxicología
1995
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| Online Access: | http://hdl.handle.net/10261/295220 |
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