In vitro immunostimulants for optimal responses of kidney cells from healthy trout and from trout surviving viral haemorrhagic septicaemia virus disease
The specific immune memory of trout surviving a double infection with viral haemorrhagic septicaemia virus (VHSV) was studied by culturing cells from the anterior kidney both in liquid and in a semisolid fibrin medium in the presence of purified proteins from VHSV. In addition, polyclonal stimulators of the immune response (phytohaemagglutinin, Concanavalin A, lipopolysaccharides and a panel of MAbs anti-trout serum immunoglobulin) were studied in healthy trout by culturing cells from the anterior kidney. Finally, an immunisation was stimulated by using simultaneous addition of both specific and unspecific immunostimulants to healthy trout kidney cells. The results suggest the necessity of employing both mitogens and viral nucleo and glycoproteins in subunit immunisation trials, and the opportunity to use this or similar in vitro assays to select for the proper epitopes in genetically engineered proteins during subunit vaccine development. © 1992 Academic Press Ltd.
| Main Authors: | , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
1992
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| Subjects: | Immunostimulants, Trout kidney cells, Viral haemorrhagic septicaemia, In vitro, |
| Online Access: | http://hdl.handle.net/20.500.12792/5054 http://hdl.handle.net/10261/294871 |
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