Towards a multi-site synthetic vaccine to foot-and-mouth disease Addition of discontinuous site peptide mimic increases the neutralization response in immunized animals
Synthetic replicas of both antigenic sites A and D of foot-and-mouth disease virus have been tested as a first step towards a multicomponent peptide vaccine candidate. A first evaluation has been performed by neutralization assays on cells with serum mixtures from guinea pigs immunized independently with site A (A24) and site D (D8) peptides. The addition of site D antibodies to site A antibodies has a synergistic effect on neutralization. In a second group of experiments, guinea pigs have been immunized with a dendrimeric tetravalent (MAP) presentation of site A peptide, alone or in combination with D8, using the same total peptide dose. While the first inoculation gives a preferential response to site A-only antigen, specific response to site D and global neutralization levels significantly increase after reimmunization, reflecting a synergistic effect of site D. © 2004 Elsevier Ltd. All rights reserved.
| Main Authors: | , , , , , |
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| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2004
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| Subjects: | Multi-epitope peptide vaccines, Discontinuous, Dendrimeric presentation, |
| Online Access: | http://hdl.handle.net/10261/294746 |
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