Predominance and persistence of a single clone of Listeria ivanovii in a Manchego cheese factory over 6 months

In an attempt to study the diversity and persistence of molecular subtypes of pathogenic Listeria spp. in a cheese factory at the La Mancha region of Spain, 43 samples were taken from incoming raw milk (cow's, ewe's, goat's and mixed species) and from certain food-contact and environmental surfaces before andor after sanitation. Of these samples, 12 contained pathogenic Listeria. From the chromogenic agar plates corresponding to those, 46 phosphatidylinositol- specific phospholipase C-positive isolates were randomly taken for further analysis, including biochemical tests and pulsed-field gel electrophoresis (PFGE). They coincided in identifying all the 46 as Listeria ivanovii subsp. ivanovii, apparently a single PFGE type. Both ewe's and goat's raw milk batches from asymptomatic animals tested along the 6-month period persistently carried the same strain, which was also obtained from inner surfaces of raw milk truck tanks and the milk dump tank at the cheese factory. Biofilm-forming abilities of this L. ivanovii clone and interference against L. monocytogenes Scott A reference strain were tested, but failed to account for the clone's apparent pervasive presence. © 2009 Blackwell Verlag GmbH.

Bibliographic Details

Main Authors:	Vázquez-Villanueva, J., Orgaz, B., Ortiz, S., López, V., Martínez Suarez, Joaquín V, Sanjose, C.
Format:	artículo biblioteca
Language:	English
Published:	John Wiley & Sons 2010
Subjects:	Listeria ivanovii, PFGE typing, Ewe’s and goat’s raw milk, Biofilms, Cheesery,
Online Access:	http://hdl.handle.net/20.500.12792/4664 http://hdl.handle.net/10261/294724
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